glutathione t gsh  (Elabscience Biotechnology)

Journal: Materials Today Bio

Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

doi: 10.1016/j.mtbio.2026.102965

Figure Lengend Snippet: Erastin treatment mimicked the ferroptosis phenotypes observed in cryopreserved ovarian tissue. (A) Immunohistochemical staining of GPX4 and ACSL4 in fresh ovarian tissue (Ctrl), cryopreserved tissue (Cryo), and fresh tissue treated with the ferroptosis inducer erastin (Ers). Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining in each group ( n = 8). (C) Measurement of Fe 2+ concentrations by colorimetric assay in the Ctrl, Cryo, and Ers groups ( n = 6). (D) Quantification of GSH levels in ovarian tissues via a colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY staining in ovarian tissues. Scale bar, 50 μm. (F) Quantification of lipid peroxidation expressed as the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 5). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The homogenate was centrifuged at 10000× g for 10 min at 4 °C, and the clear supernatants were collected and analyzed via the Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit (Elabscience, China).

Techniques: Immunohistochemical staining, Staining, Colorimetric Assay, Fluorescence

Journal: Materials Today Bio

Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

doi: 10.1016/j.mtbio.2026.102965

Figure Lengend Snippet: UC-MSCs alleviated cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 8). (C) Measurement of the Fe 2+ concentration in ovarian tissues via a colorimetric assay ( n = 6). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) CD31 immunohistochemical staining was used to assess microvessel density in ovarian tissue. Scale bar, 50 μm. (F) Quantitative analysis of CD31-positive microvessel density ( n = 5). (G) RT-qPCR analysis of the expression of angiogenesis-related genes, including VEGF, ANG2, and IGF1 ( n = 5). (H) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (I) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. Student's t -test or one-way ANOVA with LSD post hoc tests was used. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The homogenate was centrifuged at 10000× g for 10 min at 4 °C, and the clear supernatants were collected and analyzed via the Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit (Elabscience, China).

Techniques: Immunohistochemical staining, Staining, Concentration Assay, Colorimetric Assay, Quantitative RT-PCR, Expressing, Fluorescence

Journal: Materials Today Bio

Article Title: Umbilical cord mesenchymal stem cells and their extracellular vesicles attenuate cryopreservation-induced ovarian injury via the suppression of ferroptosis in an in vitro culture system

doi: 10.1016/j.mtbio.2026.102965

Figure Lengend Snippet: MSC-EVs repaired cryopreservation-induced ovarian injury by inhibiting ferroptosis. (A) Immunohistochemical staining of GPX4 and ACSL4 in the four experimental groups. Scale bar, 50 μm. (B) Quantification of the mean optical density for GPX4 and ACSL4 staining ( n = 5). (C) Quantification of Fe 2+ concentrations in ovarian tissue via a colorimetric assay ( n = 5). (D) Quantification of GSH levels via colorimetric assay ( n = 5). (E) Representative confocal images of oxidized (green) and reduced (red) C11-BODIPY. Scale bar, 50 μm. (F) Quantification of the oxidized/reduced C11-BODIPY fluorescence ratio ( n = 3). Data are presented as mean ± SD. One-way ANOVA followed by LSD post hoc test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The homogenate was centrifuged at 10000× g for 10 min at 4 °C, and the clear supernatants were collected and analyzed via the Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit (Elabscience, China).

Techniques: Immunohistochemical staining, Staining, Colorimetric Assay, Fluorescence